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2021-06-18T13:39:04+09:00
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Ritsumeikan Univ.KO-360
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BT
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/TT0 1 Tf
14.25 0 0 14.25 70.8538 788.25 Tm
(Temperature and Pressure Effects on Conformational Equilibria of Model)Tj
-1.462 -1.211 Td
(Compounds of Proteins in Aqueous Solutions: A Vibrational Spectroscopic \
Study)Tj
/TT1 1 Tf
12 0 0 12 470.3711 742.5 Tm
(Takahiro Takekiyo)Tj
-35.489 -2.313 Td
(The hydration is an important factor for the conformational equilibria o\
f simple organic molecules, such as)Tj
-1 -1.5 Td
(haloalkane and haloacetone. The hydration is also an important factor fo\
r the structural stability of proteins in)Tj
0 -1.5 TD
(aqueous solutions. However, the detail relationship between the structur\
al stability of proteins and hydration)Tj
T*
(is not clarified due to the complicated structure of proteins. Oligopept\
ides are located between the simple)Tj
T*
(organic molecules and the biopolymers such as proteins. The study of the\
hydration on conformational)Tj
T*
(equilibria of oligopeptides is important to expand the thermodynamic int\
erpretation for the hydration of)Tj
T*
(simple organic molecules. Moreover, the study of the hydration on confor\
mational equilibria of simple)Tj
T*
(organic molecules and oligopeptides could also provide us with basic inf\
ormation for understanding the)Tj
T*
(hydration effect on structural stability of proteins. However, the therm\
odynamic studies of the hydration on)Tj
T*
(conformational equilibria of model compounds of proteins including oligo\
peptides have been hardly done. In)Tj
T*
(this study, we measured the temperature and pressure effects on conforma\
tional equilibria of model)Tj
T*
(compounds of proteins in aqueous solution by using vibrational spectrosc\
opy \(Infrared and Raman\), and)Tj
T*
(determined the difference in the partial molar enthalpy \()Tj
/C2_0 1 Tf
22.435 0 Td
<01FB>Tj
/TT2 1 Tf
(H)Tj
/TT1 1 Tf
( \)and the difference in the partial molar volume)Tj
-22.435 -1.5 Td
(\()Tj
/C2_0 1 Tf
<01FB>Tj
/TT2 1 Tf
(V)Tj
/TT1 1 Tf
( \) between the conformers. From obtained )Tj
/C2_0 1 Tf
18.581 0 Td
<01FB>Tj
/TT2 1 Tf
(H)Tj
/TT1 1 Tf
( and )Tj
/C2_0 1 Tf
<01FB>Tj
/TT2 1 Tf
(V,)Tj
/TT1 1 Tf
( it was found that the contribution of the hydration)Tj
-18.581 -1.5 Td
(for )Tj
/C2_0 1 Tf
<01FB>Tj
/TT2 1 Tf
(H)Tj
/TT1 1 Tf
( and )Tj
/C2_0 1 Tf
<01FB>Tj
/TT2 1 Tf
(V)Tj
/TT1 1 Tf
( of model compounds increases with increasing chain length of model comp\
ound. Moreover,)Tj
T*
(from the pressure effect on the helix-coil transition of the helix forme\
d oligopeptide without tertiary structure)Tj
T*
(such as hydrophobic interaction, the population of the helix conformer i\
ncreases with increasing pressure, and)Tj
T*
(that of the coil conformer decreases. Our result is different from the p\
ressure effect on the helix structure in)Tj
T*
(proteins, which the helix structure in proteins breaks with increasing p\
ressure. From these results, the helix)Tj
T*
(formation oligopeptide having no tertiary structure stabilized under hig\
h pressure. Therefore, we suggested)Tj
T*
(that the tertiary structure such as hydrophobic interaction contributes \
to the pressure denaturation of proteins.)Tj
ET
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